LOCUS       EF1a-RLuc-F2A-GF        5795 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
FEATURES             Location/Qualifiers
     LTR             5561..5795
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    4882..5478
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     intron          2126..3068
                     /label=intron
     CDS             4081..4146
                     /label=F2A
     CDS             4147..4866
                     /label=GFP
     CDS             3136..4065
                     /label=Renilla\Luciferase\(RLuc)
BASE COUNT     1403 a      1483 c      1629 g      1280 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccggtt 
     3121 aacggatccg ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc 
     3181 actgggcctc agtggtgggc tcgctgcaag caaatgaacg tgctggactc cttcatcaac 
     3241 tactatgatt ccgagaagca cgccgagaac gccgtgattt ttctgcatgg taacgctgcc 
     3301 tccagctacc tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc 
     3361 cctgatctga tcggaatggg taagtccggc aagagcggga atggctcata tcgcctcctg 
     3421 gatcactaca agtacctcac cgcttggttc gagctgctga accttccaaa gaaaatcatc 
     3481 tttgtgggcc acgactgggg ggcttgtctg gcctttcact actcctacga gcaccaagac 
     3541 aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag 
     3601 tggcctgaca tcgaggagga tatcgccctg atcaagagcg aagagggcga gaaaatggtg 
     3661 cttgagaata acttcttcgt cgagaccatg ctcccaagca agatcatgcg gaaactggag 
     3721 cctgaggagt tcgctgccta cctggagcca ttcaaggaga agggcgaggt tagacggcct 
     3781 accctctcct ggcctcgcga gatccctctc gttaagggag gcaagcccga cgtcgtccag 
     3841 attgtccgca actacaacgc ctaccttcgg gccagcgacg atctgcctaa gatgttcatc 
     3901 gagtccgacc ctgggttctt ttccaacgct attgtcgagg gagctaagaa gttccctaac 
     3961 accgagttcg tgaaggtgaa gggcctccac ttcagccagg aggacgctcc agatgaaatg 
     4021 ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga acgaggctag cggcgatatc 
     4081 gtgaagcaga ccctgaactt cgacctcctg aagctggccg gcgacgtgga aagcaacccc 
     4141 gggcctatgg tgtccaaggg cgaggaactg ttcaccggcg tggtgcccat cctggtggaa 
     4201 ctggatggcg acgtgaacgg ccacaagttc agcgtgtccg gcgagggcga aggcgacgcc 
     4261 acatatggca agctgacact gaagttcatc tgcaccaccg gcaagctgcc cgtgccttgg 
     4321 cctaccctcg tgaccacact gacctacggc gtgcagtgct tctccagata ccccgaccat 
     4381 atgaagcagc acgacttctt caagagcgcc atgcccgagg gctacgtgca ggaacggacc 
     4441 atcttcttta aggacgacgg caactacaag accagggccg aagtgaagtt cgagggcgac 
     4501 accctcgtga accggatcga gctgaagggc atcgacttca aagaggacgg caacatcctg 
     4561 ggccacaagc tggagtacaa ctacaacagc cacaacgtct acatcatggc cgacaagcag 
     4621 aaaaacggca tcaaagtgaa cttcaagatc cggcacaaca tcgaggacgg ctccgtgcag 
     4681 ctggccgacc actaccagca gaacaccccc atcggagatg gccccgtgct gctgcccgac 
     4741 aaccactacc tgagcacaca gagcgccctg agcaaggacc ccaacgagaa gcgggaccac 
     4801 atggtgctgc tggagttcgt gaccgccgct ggcatcaccc tgggcatgga cgagctgtac 
     4861 aagtgaacta gtgcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 
     4921 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     4981 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     5041 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
     5101 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
     5161 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
     5221 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
     5281 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 
     5341 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
     5401 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
     5461 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc 
     5521 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 
     5581 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 
     5641 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 
     5701 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 
     5761 cagacccttt tagtcagtgt ggaaaatctc tagca 
//
